Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone

Sakurai A.; Hasegawa T.; Kudo A.; Shen Z.; Nagai T.; Abe M.; Yoshida T.; Hongo H.; Yamamoto T.; Yamamoto T.; Oda K.; Freitas de PHL.; Li M.; Sano H.; Amizuka N.

タイトル	en Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
作成者	en Sakurai, A. en Hasegawa, T. NRID 1000050739349 en Kudo, A. en Shen, Z. en Nagai, T. en Abe, M. en Yoshida, T. en Hongo, H. en Yamamoto, T. en Yamamoto, T. NRID 1000080200822 en Oda, K. NRID 1000010122681 en Freitas, de PHL. en Li, M. NRID 1000060447612 en Sano, H. NRID 1000090205998 en Amizuka, N. NRID 1000030242431
アクセス権	open access
主題	NDC 497
内容注記	Abstract en To assess the chronological participation of sclerostin and FGF23 in bone metabolism, this study tracked the immunolocalization of sclerostin and FGF23 in the metaphyses of murine long bones from embryonic day 18 (E18) through 1 day after birth, 1 week, 2 weeks, 4 weeks, 8 weeks, and 20 weeks of age. We have selected two regions in the metaphyseal trabeculae for assessing sclerostin and FGF23 localization: close to the chondro-osseous junction, i.e., bone modeling site even in the adult animals, and the trabecular region distant from the growth plate, where bone remodeling takes place. As a consequence, sclerostin-immunopositive osteocytes could not be observed in both close and distant trabecular regions early at the embryonic and young adult stages. However, osteocytes gradually started to express sclerostin in the distant region earlier than in the close region of the trabeculae. Immunoreactivity for FGF23 was observed mainly in osteoblasts in the early stages, but detectable in osteocytes in the later stages of growth in trabecular and cortical bones. Fgf23 was weakly expressed in the embryonic and neonatal stages, while the receptors, Fgfr1c and αKlotho were strongly expressed in femora. At the adult stages, Fgf23 expression became more intense while Fgfr1c and aKlotho were weakly expressed. These findings suggest that sclerostin is secreted by osteocytes in mature bone undergoing remodeling while FGF23 is synthesized by osteoblasts and osteocytes depending on the developmental/growth stage. In addition, it appears that FGF23 acts in an autocrine and paracrine fashion in fetal and neonatal bones.
出版者	en Biomedical Research Press
日付	Issued2017-08-01
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/72290
関連	isIdenticalTo DOI https://doi.org/10.2220/biomedres.38.257 PMID 28794403
収録誌情報	PISSN 0388-6107 EISSN 1880-313X NCID AA00110128 en Biomedical Research 巻38 号4 開始ページ257 終了ページ267
ファイル	fulltext A35_38_257.pdf 12.83 MB (application/pdf) Issued2017-08-01
コンテンツ更新日時	2023-07-26