Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone

Sakurai A.; Hasegawa T.; Kudo A.; Shen Z.; Nagai T.; Abe M.; Yoshida T.; Hongo H.; Yamamoto T.; Yamamoto T.; Oda K.; Freitas de PHL.; Li M.; Sano H.; Amizuka N.

Title	en Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
Creator	en Sakurai, A. en Hasegawa, T. NRID 1000050739349 en Kudo, A. en Shen, Z. en Nagai, T. en Abe, M. en Yoshida, T. en Hongo, H. en Yamamoto, T. en Yamamoto, T. NRID 1000080200822 en Oda, K. NRID 1000010122681 en Freitas, de PHL. en Li, M. NRID 1000060447612 en Sano, H. NRID 1000090205998 en Amizuka, N. NRID 1000030242431
Accessrights	open access
Subject	NDC 497
Description	Abstract en To assess the chronological participation of sclerostin and FGF23 in bone metabolism, this study tracked the immunolocalization of sclerostin and FGF23 in the metaphyses of murine long bones from embryonic day 18 (E18) through 1 day after birth, 1 week, 2 weeks, 4 weeks, 8 weeks, and 20 weeks of age. We have selected two regions in the metaphyseal trabeculae for assessing sclerostin and FGF23 localization: close to the chondro-osseous junction, i.e., bone modeling site even in the adult animals, and the trabecular region distant from the growth plate, where bone remodeling takes place. As a consequence, sclerostin-immunopositive osteocytes could not be observed in both close and distant trabecular regions early at the embryonic and young adult stages. However, osteocytes gradually started to express sclerostin in the distant region earlier than in the close region of the trabeculae. Immunoreactivity for FGF23 was observed mainly in osteoblasts in the early stages, but detectable in osteocytes in the later stages of growth in trabecular and cortical bones. Fgf23 was weakly expressed in the embryonic and neonatal stages, while the receptors, Fgfr1c and αKlotho were strongly expressed in femora. At the adult stages, Fgf23 expression became more intense while Fgfr1c and aKlotho were weakly expressed. These findings suggest that sclerostin is secreted by osteocytes in mature bone undergoing remodeling while FGF23 is synthesized by osteoblasts and osteocytes depending on the developmental/growth stage. In addition, it appears that FGF23 acts in an autocrine and paracrine fashion in fetal and neonatal bones.
Publisher	en Biomedical Research Press
Date	Issued2017-08-01
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/72290
Relation	isIdenticalTo DOI https://doi.org/10.2220/biomedres.38.257 PMID 28794403
Journal	PISSN 0388-6107 EISSN 1880-313X NCID AA00110128 en Biomedical Research Volume Number38 Issue Number4 Page Start257 Page End267
File	fulltext A35_38_257.pdf 12.83 MB (application/pdf) Issued2017-08-01
Oaidate	2023-07-26