Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats

Hasegawa T.; Li M.; Hara K.; Sasaki M.; Tabata C.; Freitas PHL.; Hongo H.; Suzuki R.; Kobayashi M.; Inoue K.; Yamamoto T.; Oohata N.; Oda K.; Akiyama Y.; Amizuka N.

タイトル	en Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats
作成者	en Hasegawa, T. NRID 1000050739349 en Li, M. NRID 1000060447612 en Hara, K. en Sasaki, M. en Tabata, C. en Freitas, PHL. en Hongo, H. en Suzuki, R. NRID 1000090333723 en Kobayashi, M. en Inoue, K. NRID 1000030168439 en Yamamoto, T. NRID 1000080200822 en Oohata, N. en Oda, K. NRID 1000010122681 en Akiyama, Y. en Amizuka, N. NRID 1000030242431
アクセス権	open access
主題	NDC 497
内容注記	Abstract en Osteogenic disorder shionogi (ODS) rats carry a hereditary defect in ascorbic acid synthesis, mimicking human scurvy when fed with an ascorbic acid-deficient (aa-def) diet. As aa-def ODS rats were shown to feature disordered bone formation, we have examined the bone mineralization in this rat model. A fibrous tissue layer surrounding the trabeculae of tibial metaphyses was found in aa-def ODS rats, and this layer showed intense alkaline phosphatase activity and proliferating cell nuclear antigen-immunopositivity. Many osteoblasts detached from the bone surfaces and were characterized by round-shaped rough endoplasmic reticulum (rER), suggesting accumulation of malformed collagen inside the rER. Accordingly, fine, fragile fibrillar collagenous structures without evident striation were found in aa-def bones, which may result from misassembling of the triple helices of collagenous α-chains. Despite a marked reduction in bone formation, ascorbic acid deprivation seemed to have no effect on mineralization: while reduced in number, normal matrix vesicles and mineralized nodules could be seen in aa-def bones. Fine needle-like mineral crystals extended from these mineralized nodules, and were apparently bound to collagenous fibrillar structures. In summary, collagen mineralization seems unaffected by ascorbic acid deficiency in spite of the fine, fragile collagenous fibrils identified in the bones of our animal model.
出版者	en Biomedical Research Press
日付	Issued2011-08-30
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/72292
関連	isIdenticalTo DOI https://doi.org/10.2220/biomedres.32.259
収録誌情報	PISSN 0388-6107 EISSN 1880-313X NCID AA00110128 en Biomedical Research 巻32 号4 開始ページ259 終了ページ269
ファイル	fulltext A4_32_259.pdf 1.6 MB (application/pdf) Issued2011-08-30
コンテンツ更新日時	2023-07-26