Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats

Hasegawa T.; Li M.; Hara K.; Sasaki M.; Tabata C.; Freitas PHL.; Hongo H.; Suzuki R.; Kobayashi M.; Inoue K.; Yamamoto T.; Oohata N.; Oda K.; Akiyama Y.; Amizuka N.

Title	en Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats
Creator	en Hasegawa, T. NRID 1000050739349 en Li, M. NRID 1000060447612 en Hara, K. en Sasaki, M. en Tabata, C. en Freitas, PHL. en Hongo, H. en Suzuki, R. NRID 1000090333723 en Kobayashi, M. en Inoue, K. NRID 1000030168439 en Yamamoto, T. NRID 1000080200822 en Oohata, N. en Oda, K. NRID 1000010122681 en Akiyama, Y. en Amizuka, N. NRID 1000030242431
Accessrights	open access
Subject	NDC 497
Description	Abstract en Osteogenic disorder shionogi (ODS) rats carry a hereditary defect in ascorbic acid synthesis, mimicking human scurvy when fed with an ascorbic acid-deficient (aa-def) diet. As aa-def ODS rats were shown to feature disordered bone formation, we have examined the bone mineralization in this rat model. A fibrous tissue layer surrounding the trabeculae of tibial metaphyses was found in aa-def ODS rats, and this layer showed intense alkaline phosphatase activity and proliferating cell nuclear antigen-immunopositivity. Many osteoblasts detached from the bone surfaces and were characterized by round-shaped rough endoplasmic reticulum (rER), suggesting accumulation of malformed collagen inside the rER. Accordingly, fine, fragile fibrillar collagenous structures without evident striation were found in aa-def bones, which may result from misassembling of the triple helices of collagenous α-chains. Despite a marked reduction in bone formation, ascorbic acid deprivation seemed to have no effect on mineralization: while reduced in number, normal matrix vesicles and mineralized nodules could be seen in aa-def bones. Fine needle-like mineral crystals extended from these mineralized nodules, and were apparently bound to collagenous fibrillar structures. In summary, collagen mineralization seems unaffected by ascorbic acid deficiency in spite of the fine, fragile collagenous fibrils identified in the bones of our animal model.
Publisher	en Biomedical Research Press
Date	Issued2011-08-30
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/72292
Relation	isIdenticalTo DOI https://doi.org/10.2220/biomedres.32.259
Journal	PISSN 0388-6107 EISSN 1880-313X NCID AA00110128 en Biomedical Research Volume Number32 Issue Number4 Page Start259 Page End269
File	fulltext A4_32_259.pdf 1.6 MB (application/pdf) Issued2011-08-30
Oaidate	2023-07-26