Title |
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Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 K
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en
Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 K
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Creator |
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Accessrights |
metadata only access |
Rights |
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© 2011 American Physical Society
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Description |
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Abstract
The visible fluorescence spectrum of single flavoprotein at a temperature of 1.5 K has been measured by one-photon excitation. The flavoprotein studied was a photoswitchable enzyme, photoactivated adenylyl cyclase. The time course of the spectrum revealed a structural change occurring at a rate of 10-3 s-1 around hydrogen bonds at the flavin cofactor binding site.
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Publisher |
American Physical Society
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Date |
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Language |
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Resource Type |
journal article |
Identifier |
URI
https://ir.soken.ac.jp/records/3331
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Relation |
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DOI
https://doi.org/10.1103/PhysRevLett.106.078101
10.1103/PhysRevLett.106.078101
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Journal |
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Physical Review Letters
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Physical Review Letters
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Volume Number106
Issue Number7
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Oaidate |
2023-08-18 |