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Title
  • THE EFFECTIVENESS OF TRYPSIN TREATMENT TO REMOVE SENDAI VIRUS ADHERING TO THE ZONA PELLUCIDA OF MOUSE PREIMPLANTATION EMBRYOS
Creator

LAVILLA-APELO, Carmencita

KIDA, Hiroshi

NRID 1000010109506

KANAGAWA, Hiroshi

Subject
  • Other mouse embryo
  • Other Sendai virus
  • Other washing
  • Other trypsin treatment
  • NDC 649
Description
Other
  • The standard washing and trypsin treatment procedures to remove viruses adhering to the zona pellucida (ZP) were evaluated. Mouse embryos at the early blastocyst stage were exposed to Sendai virus, and then washed or treated with trypsin. Even after washing or trypsin treatment, Sendai virus was detected in the twelfth and final wash. The virus was still shown to adhere to the ZP by immunofluorescence assay. The embryos developed into expanded blastocysts following 24 hours of in vitro culture. Viral antigen was clearly demonstrated in the cells forming the expanded blastocysts, indicating that viral replication occurred in these cells. The present results suggest that the standard washing or trypsin treatment are not sufficient to remove Sendai virus adhering to the ZP of mouse embryos.
PublisherThe Graduate School of Veterinary Medicine
Date Issued 1991-12-26
Languageeng
NIItypedepartmental bulletin paper
VersiontypeVoR
Identifier URI http://hdl.handle.net/2115/8937 , DOI https://doi.org/10.14943/jjvr.39.2-4.133
ID

JaLC10.14943/jjvr.39.2-4.133

Journal
    • NCID AA0069128X
    • ISSN 0047-1917
    • Japanese Journal of Veterinary Research
    39(2-4), 133-141
File
Oaidate2019-04-25T02:11:39Z