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Title
  • en Characterization of mouse tissue kallikrein 5
Creator
    • en Rajapakse, Sanath
    • en Yamano, Noriko
    • en Hirata, Kensaku
    • en Takahashi, Sumio
Accessrights open access
Subject
  • Other en mouse
  • Other en protease
  • Other en kallikrein 5
  • Other en recombinant enzyme
  • Other en characterization
  • NDC 481.35
Description
  • Abstract en Mouse tissue kallikreins (Klks) are members of a large, multigene family consisting of 37 genes, 26 of which can code for functional proteins. Mouse tissue kallikrein 5 (Klk5) has long been thought to be one of these functional genes, but the gene product, mK5, has not been isolated and characterized. In the present study, we prepared active recombinant mK5 using an Escherichia coli expression system, followed by column chromatography. We then determined the biochemical and enzymatic properties of purified mK5. mK5 had trypsin-like activity for Arg at the P1 position, and its activity was inhibited by typical serine protease inhibitors. mK5 degraded gelatin, fibronectin, collagen type IV, high-molecular-weight kininogen, and insulin-like growth factor binding protein-3. Our data suggest that mK5 may be implicated in the process of extracellular matrix remodeling.
Publisher en Zoological Society of Japan
Date
    Issued2006-11
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/18640
Relation
  • isVersionOf DOI https://doi.org/10.2108/zsj.23.963
  • PMID 17189908
Journal
    • PISSN 0289-0003
      • en ZOOLOGICAL SCIENCE
      • Volume Number23 Issue Number11 Page Start963 Page End968
File
    • fulltext ZS23-11.pdf
    • 527.88 KB (application/pdf)
      • Issued2006-11
Oaidate 2023-07-26