| Title |
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Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells
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| Creator |
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| Accessrights |
open access |
| Rights |
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en
The original publication is available at
springerlink.com
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| Subject |
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Other
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Fish serum
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Other
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Chinese hamster ovary cells
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Other
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Lipid
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Other
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Heat treatment
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Other
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Low concentration
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NDC
463
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| Description |
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Abstract
en
The effects of heat treatment and concentration of fish serum (FS) on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of recombinant Chinese hamster ovary (CHO) cells, DR1000L4N, were investigated. The addition of heat treated FS instead of non-heat-treated FS improved cell growth in terms of cell density, which reached 60% that in 10% fetal calf serum (FCS)-containing medium (FCS medium). A decrease in FS concentration from 10% to 1.25% markedly increased cell density, which was 79% that in 10% FCS medium. The combination of heat treatment at 56℃ C and the addition of FS at a low concentration (1.25%) showed an additive effect on cell growth and resulted in the same cell density as that in 10% FCS medium, whereas the hGM-CSF concentration in the culture using FS-containing medium (FS medium) was approximately 50% that in 10% FCS medium. The total lipid concentration in FS was more than three fold that in FCS. The effect of decreasing FS concentration on cell growth may be due to the low lipid concentration in FS medium, because addition of the lipids extracted from FS to 10% FCS and 1.25% FS media markedly decreased cell density. Consequently, the addition of heat-treated FS at low concentrations to medium may be useful for the growth of CHO cells without FCS.
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| Publisher |
en
Springer Netherlands
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| Date |
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| Language |
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| Resource Type |
journal article |
| Version Type |
AM |
| Identifier |
HDL
http://hdl.handle.net/2115/38750
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| Relation |
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isVersionOf
DOI
https://doi.org/10.1007/s10616-009-9199-7
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| Journal |
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Cytotechnology
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Volume Number59
Issue Number2
Page Start135
Page End141
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| File |
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| Oaidate |
2023-07-26 |
