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Title
  • en Visualization of Nuclear Localization of Transcription Factors with Cyan and Green Fluorescent Proteins in the Red Alga Porphyra yezoensis
Creator
Accessrights open access
Subject
  • Other en Transcription factor
  • Other en nuclear localization
  • Other en fluorescent protein
  • Other en transient gene expression
  • Other en Porphyra yezoensis
  • NDC 450
Description
  • Abstract en Transcription factors play a central role in expression of genomic information in all organisms. The objective of our study is to analyze the function of transcription factors in red algae. One way to analyze transcription factors in eukaryotic cells is to study their nuclear localization, as reported for land plants and green algae using fluorescent proteins. There is, however, no report documenting subcellular localization of transcription factors from red algae. In the present study, using the marine red alga Porphyra yezoensis, we confirmed for the first time successful expression of humanized fluorescent proteins (ZsGFP and ZsYFP) from a reef coral Zoanthus sp. and land plant-adapted sGFP(S65T) in gametophytic cells comparable to expression of AmCFP. Following molecular cloning and characterization of transcription factors DP-E2F-like 1 (PyDEL1), transcription elongation factor 1 (PyElf1) and multiprotein bridging factor 1 (PyMBF1), we then demonstrated that ZsGFP and AmCFP can be used to visualize nuclear localization of PyElf1 and PyMBF1. This is the first report to perform visualization of subcellular localization of transcription factors as genome-encoded proteins in red algae.
Publisher en Springer
Date
    Issued2010-04
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/42987
Relation
  • isVersionOf DOI https://doi.org/10.1007/s10126-009-9210-5
  • PMID 19593603
Journal
    • PISSN 1436-2228
    • EISSN 1436-2236
      • en Marine Biotechnology
      • Volume Number12 Issue Number2 Page Start150 Page End159
File
    • fulltext uji_et_al.pdf
    • 510.18 KB (application/pdf)
      • Issued2010-04
Oaidate 2023-07-26