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Title
  • en Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis
Creator
    • en Fukuda, Satoru
    • en Park, Eun-Jeong
    • en Ohba, Toshiharu
    • en Asada, Kiyozo
    • en Kitade, Yukihiro
    • en Endo, Hirotoshi
    • en Kato, Ikunoshin
Accessrights open access
Subject
  • Other en Porphyra yezoensis
  • Other en Codon usage
  • Other en β-Glucuronidase (EC 3.2.1.21)
  • Other en Particle bombardment
  • Other en Transient gene expression
  • NDC 474
Description
  • Abstract en The marine red alga Porphyra yezoensis has been proposed as a model plant for physiological and genetic studies in seaweeds because of its biological and economical importance. However, the progress of molecular biological studies using gene transfection and genetic transformation systems has been hindered by difficulties in the expression of foreign genes in P. yezoensis cells. To overcome this situation, we developed a transient gene expression system to monitor gene expression in P. yezoensis cells. An artificial υ-glucuronidase (GUS) coding region was synthesized to adapt it to the codon usage of P. yezoensis (PyGUS) and then evaluated for efficiency as a reporter of transient gene expression by particle bombardment. We also demonstrated the importance of using the promoter of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from P. yezoensis for efficient expression of PyGUS, because the cauliflower mosaic virus (CaMV) 35S promoter, which has been successfully used for monitoring gene expression in nuclei and chloroplasts of higher plants, was less active in P. yezoensis cells. Therefore, the lack of knowledge about differences in the regulatory machinery of gene expression between P. yezoensis and terrestrial plants seems to be why experimental systems for monitoring gene expression were previously not developed in P. yezoensis. Establishment of the transient gene expression system in P. yezoensis could facilitate biotechnological developments in this organism.
Publisher en Elsevier
Date
    Issued2008-03
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/39214
Relation
  • isVersionOf DOI https://doi.org/10.1016/j.plantsci.2007.12.006
Journal
    • PISSN 0168-9452
    • NCID AA10531279
      • en Plant Science : an international journal of experimental plant biology
      • Volume Number174 Issue Number3 Page Start329 Page End339
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Oaidate 2023-07-26