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Title
  • en Two Related but Distinct Chondroitin Sulfate Mimetope Octasaccharide Sequences Recognized by Monoclonal Antibody WF6
Creator
    • en Pothacharoen, Peraphan
    • en Kalayanamitra, Kittiwan
    • en Deepa, Sarama
    • en Fukui, Shigeyuki
    • en Hattori, Tomohide
    • en Fukushima, Nobuhiro
    • en Hardingham, Timothy
    • en Kongtawelert, Prachya
Accessrights open access
Subject
  • Other en chondroitin sulfate
  • Other en epitope
  • Other en monoclonal antibody
  • Other en octasaccharides
  • Other en sulfation
  • Other en molecular modeling
  • NDC 460
Description
  • Abstract en Chondroitin sulfate (CS) proteoglycans are major components of cartilage and other connective tissues. The monoclonal antibody (mAb) WF6, developed against embryonic shark cartilage CS, recognizes an epitope in CS chains, which is expressed in ovarian cancer and variably in joint diseases. To elucidate the structure of the epitope, we isolated oligosaccharide fractions from a partial chondroitinase ABC digest of shark cartilage CS-C and established their chain length, disaccharide composition, sulfate content and sulfation pattern. These structurally defined oligosaccharide fractions were characterized for binding to WF6 by enzyme-linked immunosorbent assay using an oligosaccharide microarray prepared with CS oligosaccharides derivatized with a fluorescent aminolipid. The lowest molecular weight fraction recognized by WF6 contained octasaccharides, which were split into five subfractions. The most reactive subfraction contained several distinct octasaccharide sequences. Two octasaccharides, **」GD-C-C-C and 」GC-C-A-D, were recognized by WF6, but other related octasaccharides, 」GC-A-D-C and 」GC-C-C-C, were not. The structure and sequences of both the binding and non-binding octasaccharides were compared by computer modeling, which revealed a remarkable similarity between the shape and distribution of the electrostatic potential in the two different octasaccharide sequences that bound to WF6 and which differed from the non-binding octasaccharides. The strong similarity in structure predicted for the two binding CS octasaccharides (」GD-C-C-C and 」GC-C-A-D) provided a possible explanation for their similar affinity for the WF6, although they differed in sequence and thus form two specific mimetopes for the antibody. **Abbreviations: A, GlcUAυ1-3GalNAc(4-O-sulfate); C, GlcUAυ1-3GalNAc(6-O-sulfate); D, GlcUA(2-O-sulfate)υ1-3GalNAc(6-O-sulfate); 」GC, 」G4,5HexUAτ1-3GalNAc(6-O-sulfate); 」GD, 」G4,5HexUA(2-O-sulfate)τ1-3GalNAc(6-O-sulfate).
Date
    Issued2007-11-30
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/44203
Relation
  • isVersionOf DOI https://doi.org/10.1074/jbc.M702255200
  • PMID 17884822
Journal
  • en Journal of Biological Chemistry
  • Volume Number282 Issue Number48 Page Start35232 Page End35246
File
Oaidate 2023-07-26