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Title
  • Knockdown of legumain inhibits cleavage of annexin A2 in the mouse kidney
Creator

Yamane, Takuya

Hachisu, Rei

Yuguchi, Motoki

Takeuchi, Keisuke

Murao, Sato

Yamamoto, Yoshio

Ogita, Hisakazu

Takasawa, Toshihide

Ohkubo, Iwao

Ariga, Hiroyoshi

Subject
  • Other Legumain
  • Other Annexin A2
  • Other Cationic liposome
  • Other siRNA
  • Other In vivo
  • Other Mouse kidney
  • NDC 491
Description
Other
  • Legumain (EC 3.4.22.34) is an asparaginyl endopeptidase. Strong legumain activity was observed in the mouse kidney, and legumain was highly expressed in tumors. We previously reported that bovine kidney annexin A2 was co-purified with legumain and that legumain cleaved the N-terminal region of annexin A2 at an Asn residue in vitro. In this study, to determine whether annexin A2 is cleaved by legumain in vivo, siRNA-lipoplex targeting mouse legumain was injected into mouse tail veins. Mouse kidneys were then isolated and the effect of knockdown of legumain expression on annexin A2 cleavage was examined. The results showed that both legumain mRNA and protein expression levels were decreased in the siRNA-treated mouse kidneys and that legumain activity toward a synthetic substrate, Z-Ala-Ala-Asn-MCA, was decreased by about 40% in the kidney but not in the liver or spleen. Furthermore, cleavage of annexin A2 at the N-terminal region was decreased in the mouse kidney that had been treated with the legumain siRNA-lipoplex. These results suggest that legumain siRNA was delivered to the kidney by using LipoTrust and that the reduced legumain expression inhibited legumain-induced degradation of annexin A2 in vivo. (C) 2012 Elsevier Inc. All rights reserved.
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Date Issued 2013-01-11
Languageeng
NIItypejournal article
VersiontypeAM
Identifier URI http://hdl.handle.net/2115/52625
Relation
  • isIdenticalTo PMID 23237799
  • isIdenticalTo DOI https://doi.org/10.1016/j.bbrc.2012.12.010
Journal
    • ISSN 0006-291X
    • BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    430(2), 482-487
File
Oaidate2019-03-15T05:20:37Z