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Title	en Analysis of salivary gland transcripts of the sand fly Lutzomyia ayacuchensis, a vector of Andean-type cutaneous leishmaniasis
Creator	en Kato, Hirotomo NRID 1000000346579 en Jochim, Ryan C. en Gomez, Eduardo A. en Uezato, Hiroshi NRID 1000060160157 en Mimori, Tatsuyuki NRID 1000000117384 en Korenaga, Masataka en Sakurai, Tatsuya en Katakura, Ken NRID 1000010130155 en Valenzuela, Jesus G. en Hashiguchi, Yoshihisa NRID 1000010037385
Accessrights	open access
Subject	Other en Lutzomyia ayacuchensis Other en Salivary gland Other en Transcript Other en Bioinformatics Other en cDNA library NDC 493
Description	Abstract en The saliva of blood sucking insects contains potent pharmacologically active components that assist them in counteracting the host hemostatic and inflammatory systems during blood feeding. In addition, sand fly salivary proteins affect host immunity and have the potential to be a vaccine against Leishmania infection. In the present study, the salivary gland transcripts of Lutzomyia (Lu.) ayacuchensis, a vector of cutaneous leishmaniasis in Ecuadorian and Peruvian Andes, were analyzed by sequencing randomly selected clones of the salivary gland cDNA library of this sand fly. This resulted in the identification of the most abundant transcripts coding for secreted proteins. These proteins were homologous to the salivary molecules present in other sand flies including the RGD-containing peptide, PpSP15/SL1 family protein, yellow-related protein, putative apyrase, antigen 5-related protein, D7 family protein, and 27 kDa salivary protein. Of note, homologues of maxadilan, an active vasodilator abundantly present in saliva of Lu. longipalpis, were not identified. This analysis is the first description of salivary proteins from a sand fly of the subgenus Helcocyrtomyia and from vector of cutaneous leishmaniasis in the New World. The present analysis will provide further insights into the evolution of salivary components in blood sucking arthropods.
Publisher	en Elsevier B.V.
Date	Issued2013-01
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/52263
Relation	isVersionOf DOI https://doi.org/10.1016/j.meegid.2012.08.024
Journal	PISSN 1567-1348 en Infection, Genetics and Evolution Volume Number13 Page Start56 Page End66
File	fulltext IGE13_56-66.pdf 706.8 KB (application/pdf) Issued2013-01 fulltext Supplementary_data.pptx 159.58 KB (application/vnd.openxmlformats-officedocument.presentationml.presentation) Issued2013-01
Oaidate	2023-07-26