Title |
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Identification of the recognition sequence and target proteins for DJ-1 protease
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Creator |
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Yoshizawa-Kumagaye, Kumiko
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Accessrights |
open access |
Subject |
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Other
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DJ-1
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Other
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Protease
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Other
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Biochemistry
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NDC
464
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Description |
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Abstract
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DJ-1, the product of familial Parkinson's disease gene and an oncogene, is a cysteine protease which plays a role in anti-oxidative stress reaction. In this study, we identified the recognition sequence for DJ-1 protease by using recombinant DJ-1 and a peptide library. Protease activity of DJ-1 lacking C-terminal alpha-helix (DJ-1 Delta H9) was stronger than that of full-sized DJ-1, and the most susceptible sequence digested by DJ-1 Delta H9 was valine-lysine-valine-alanine (VKVA) under the optimal conditions of pH 5.5 and 0 mM NaCl. Divalent ions, especially Cu2+, were inhibitory to DJ-1's protease activity. c-abl oncogene 1 product (ABL1) and kinesin family member 1B (KIF1B) containing VKVA were digested by DJ-1 Delta H9. Structured summary of protein interactions: DJ-1 cleaves IUF1B by enzymatic study (View interaction) DJ-1 cleaves ABLI by enzymatic study (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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Publisher |
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Elsevier science bv
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Date |
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Language |
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Resource Type |
journal article |
Version Type |
AM |
Identifier |
HDL
http://hdl.handle.net/2115/53264
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Relation |
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DOI
https://doi.org/10.1016/j.febslet.2013.06.032
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PMID
23831022
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Journal |
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Febs letters
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Volume Number587
Issue Number16
Page Start2493
Page End2499
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File |
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Oaidate |
2023-07-26 |