• Transcriptional regulation of the legumain gene by p53 in HCT116 cells

Yamane, Takuya

Murao, Sato

Kato-Ose, Izumi

Kashima, Lisa

Yuguchi, Motoki

Kozuka, Miyuki

Takeuchi, Keisuke

Ogita, Hisakazu

Ohkubo, Iwao

Ariga, Hiroyoshi

  • Other Legumain
  • Other p53
  • Other Doxorubicin
  • NDC 499
  • Legumain (EC is an asparaginyl endopeptidase. Strong legumain activity was observed in the mouse kidney, and legumain was found to be highly expressed in tumors. We previously reported that bovine kidney annexin A2 was co-purified with legumain and that legumain cleaved the N-terminal region of annexin A2 at an Asn residue in vitro and in vivo. In this study, we found a p53-binding site in intron 1 of the human legumain gene using computational analysis. To determine whether transcription of the legumain gene is regulated by p53, HCT116 cells were transfected with p53 siRNA and the effect of knockdown of p53 expression on legumain expression was examined. The results showed that expression levels of both legumain mRNA and protein were decreased in the siRNA-treated cells. Furthermore, enzyme activity of legumain was also increased by doxorubicin and its activity was reduced by knockdown of p53 in HCT116 cells. These results suggest that legumain expression and its enzyme activity are regulated by p53. (C) 2013 Elsevier Inc. All rights reserved.
PublisherAcademic press inc elsevier science
Date Issued 2013-09-06
NIItypejournal article
Identifier URI
  • isIdenticalTo PMID 23942113
  • isIdenticalTo DOI
    • ISSN 0006-291X
    • Biochemical and biophysical research communications
    438(4), 613-618