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Title
  • en Transcriptional regulation of the legumain gene by p53 in HCT116 cells
Creator
Accessrights open access
Subject
  • Other en Legumain
  • Other en p53
  • Other en Doxorubicin
  • NDC 499
Description
  • Abstract en Legumain (EC 3.4.22.34) is an asparaginyl endopeptidase. Strong legumain activity was observed in the mouse kidney, and legumain was found to be highly expressed in tumors. We previously reported that bovine kidney annexin A2 was co-purified with legumain and that legumain cleaved the N-terminal region of annexin A2 at an Asn residue in vitro and in vivo. In this study, we found a p53-binding site in intron 1 of the human legumain gene using computational analysis. To determine whether transcription of the legumain gene is regulated by p53, HCT116 cells were transfected with p53 siRNA and the effect of knockdown of p53 expression on legumain expression was examined. The results showed that expression levels of both legumain mRNA and protein were decreased in the siRNA-treated cells. Furthermore, enzyme activity of legumain was also increased by doxorubicin and its activity was reduced by knockdown of p53 in HCT116 cells. These results suggest that legumain expression and its enzyme activity are regulated by p53. (C) 2013 Elsevier Inc. All rights reserved.
Publisher en Academic press inc elsevier science
Date
    Issued2013-09-06
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/54547
Relation
  • isVersionOf DOI https://doi.org/10.1016/j.bbrc.2013.08.007
  • PMID 23942113
Journal
    • PISSN 0006-291X
      • en Biochemical and biophysical research communications
      • Volume Number438 Issue Number4 Page Start613 Page End618
File
Oaidate 2023-07-26