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Title
  • Transcriptional Activation of the Cholecystokinin Gene by DJ-1 through Interaction of DJ-1 with RREB1 and the Effect of DJ-1 on the Cholecystokinin Level in Mice
Creator

Yamane, Takuya

Suzui, Sayaka

Kitaura, Hirotake

Takahashi-Niki, Kazuko

Iguchi-Ariga, Sanae M. M.

Ariga, Hiroyoshi

Rights
    • http://creativecommons.org/licenses/by/3.0/
Subject
  • NDC 499
Description
Other
  • DJ-1 is an oncogene and also causative gene for familial Parkinson’s disease. DJ-1 has multiple functions, including transcriptional regulation. DJ-1 acts as a coactivator that binds to various transcription factors, resulting in stimulation or repression of the expression of their target genes. In this study, we found that the cholecystokinin (CCK) gene is a transcriptional target gene for DJ-1. CCK is a peptide hormone and plays roles in contraction of the gallbladder and in promotion of secretion of pancreatic fluid. CCK is co-localized with dopamine in the substantia nigra to regulate release of dopamine. Reduced expression of CCK mRNA was observed in DJ-1-knockdown cells. The Ras-responsive element (RRE) and Sp1 site were essential for promoter activity, and DJ-1 stimulated promoter activity by binding to RRE-binding protein 1 (RREBP1). The complex of DJ-1 with RREB1 but not with Sp1 bound to the RRE. Furthermore, the reduced CCK level in the serum from DJ-1-knockout mice compared to that from wild-type mice was observed. This is the first report showing that DJ-1 participates in peptide hormone synthesis.
PublisherPLOS
Date Issued 2013-11-05
Languageeng
NIItypejournal article
VersiontypeVoR
Identifier URI http://hdl.handle.net/2115/53695
Relation
  • isIdenticalTo DOI https://doi.org/10.1371/journal.pone.0078374
Journal
    • ISSN 1932-6203
    • PLoS ONE
    8(11), e78374
File
Oaidate2017-10-15T15:00:00Z