• Pyrroloquinoline quinone prevents oxidative stress-induced neuronal death probably through changes in oxidative status of DJ-1.

Nunome, Kana

Miyazaki, Shin

Nakano, Masahiko

Iguchi-Ariga, Sanae

Ariga, Hiroyoshi

  • Other DJ-1
  • Other pyrroloquinoline quinone
  • Other oxidative stress
  • Other neurotoxity
  • NDC 499
  • MeSH Animals
  • MeSH Antioxidants/pharmacology
  • MeSH Ascorbic Acid/pharmacology
  • MeSH Blotting, Western
  • MeSH Cell Death/drug effects
  • MeSH Cell Survival/drug effects
  • MeSH Dose-Response Relationship, Drug
  • MeSH Female
  • MeSH Hydrogen Peroxide/antagonists & inhibitors
  • MeSH Hydrogen Peroxide/toxicity
  • MeSH Microtubule-Associated Proteins/drug effects
  • MeSH Microtubule-Associated Proteins/genetics
  • MeSH Microtubule-Associated Proteins/metabolism
  • MeSH Neurons/drug effects
  • MeSH Oxidants/antagonists & inhibitors
  • MeSH Oxidants/toxicity
  • MeSH Oxidation-Reduction
  • MeSH Oxidative Stress/drug effects
  • MeSH Oxidopamine/antagonists & inhibitors
  • MeSH Oxidopamine/toxicity
  • MeSH Pregnancy
  • MeSH Protein Binding
  • MeSH Pyrroles/pharmacology
  • MeSH Quinolines/pharmacology
  • MeSH Rats
  • MeSH Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • MeSH Tetrazolium Salts
  • MeSH Thiazoles
  • MeSH Tumor Cells, Cultured
  • MeSH Vitamin E/pharmacology
  • Pyrroloquinoline quinone (PQQ) has been shown to play a role as an anti-oxidant in neuronal cells and prevent neuronal cell death in a rodent stroke model. DJ-1, a causative gene product for a familial form of Parkinson's disease, plays a role in anti-oxidative stress function by self-oxidation of DJ-1. In this study, the expression level and oxidation status of DJ-1 were examined in SHSY-5Y cells and primary cultured neurons treated with 6-hydroxydopamine (6-OHDA) or H(2)O(2) in the presence or absence of PQQ. The pI shift of DJ-1 to an acidic point, which was observed in SHSY-5Y cells treated with 6-OHDA, was inhibited by PQQ. TOF-MS analyses showed that while the level of a reduced form of DJ-1, one of the active forms of DJ-1, was decreased in SHSY-5Y cells treated with 6-OHDA or H(2)O(2), PQQ increased the level of the reduced form of DJ-1. These results suggest that PQQ prevents oxidative stress-induced changes in oxidative status of DJ-1. Therefore, the neuroprotective effects of PQQ on oxidative stress-induced neuronal death may be at least in part involved in increased level of an active form of DJ-1.
PublisherThe Pharmaceutical Society of Japan
Date Issued 2008-07
NIItypejournal article
Identifier URI
  • isIdenticalTo PMID 18591768
  • isIdenticalTo DOI
    • ISSN 0918-6158
    • Biological & pharmaceutical bulletin
    31(7), 1321-1326