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Title
  • en Cloned origin of DNA replication in c-myc gene can function and be transmitted in transgenic mice in an episomal state.
Creator
    • en Sudo, Katsuko
    • en Ogata, Masaaki
    • en Sato, Yoshinari
Accessrights open access
Subject
  • MeSH en Animals
  • MeSH en Cloning, Molecular
  • MeSH en DNA Replication
  • MeSH en Female
  • MeSH en Genes, myc
  • MeSH en Humans
  • MeSH en Male
  • MeSH en Mice
  • MeSH en Mice, Transgenic
  • MeSH en Ovum
  • MeSH en Pedigree
  • MeSH en Plasmids
  • MeSH en Restriction Mapping
  • MeSH en Spermatozoa
  • NDC 499
Description
  • Abstract en The c-myc protein has recently been shown to interact with a region possessing putative origin of DNA replication and enhancer activities located 2 kb upstream of the c-myc gene itself. Transgenic mice were obtained by injecting constructs containing this region, termed pmyc(H-P), into fertilized mouse eggs. The transgenic elements were capable of efficient replication in all mouse tissues examined and were maintained in an episomal state even in highly differentiated cells. Moreover, pmyc(H-P) was transmittable to the progeny throughout several generations, which suggests that the fragment derived from the region upstream of the c-myc gene possesses sequences necessary for partition, stability and DNA replication of the plasmid in the cells. In addition, we have shown that the plasmid might be captured only by eggs, not by sperm.
  • Other http://nar.oxfordjournals.org/content/18/18/5425.abstract
Publisher en Oxford University Press
Date
    Issued1990-09-25
Language
  • eng
Resource Type journal article
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/54048
Relation
  • isIdenticalTo DOI https://doi.org/10.1093/nar/18.18.5425
  • PMID 2216716
Journal
    • PISSN 0305-1048
      • en Nucleic acids research
      • Volume Number18 Issue Number18 Page Start5425 Page End5432
File
Oaidate 2023-07-26