| Title |
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en
Measurement of DNA damage by terminal deoxynucleotidyl transferase reaction
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| Creator |
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| Accessrights |
open access |
| Rights |
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| Subject |
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Other
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DNA Damage
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Other
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Quantification
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Other
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TUNEL
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Other
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Apoptosis
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Other
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PC12 Cells
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NDC
464
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| Description |
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Abstract
en
An improved terminal deoxynucleotidyl transferase- mediated dUTP-biotin nick end labeling method for the quantification of DNA damage in tissues and cultured cells was developed. Many reports have revealed that histochemistry of DNA damage can be visualized using immunohistochemistry for the terminal deoxynucleotidyl transferase reaction in tissue sections. However, few reports have described quantification of DNA damage in tissues or cells. In this study, to estimate the degree of DNA damage, the confirmed method for histochemistry using biotinylated dUTP and deoxynucleotidyl transferase was applied to label the cleaved DNA ends caused by DNA damage in tissues or cells. After end-labeling, avidin-conjugated peroxidase was reacted. A significant correlation was observed between numbers of cleaved DNA ends and peroxidase activity after the reaction. The obtained signals for presented method showed higher than those for ordinary method, and correlate with degree of DNA damage caused by serum deprivation and chemical dose. In addition, DNA damage caused by apoptosis in cells treated with 6-hydroxydopamine or Cu and in the tissues of rats administered a diet containing no Zn could be evaluated quantitatively using the present method.
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| Publisher |
en
Scientific Research Publishing Inc.
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| Date |
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| Language |
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| Resource Type |
journal article |
| Version Type |
VoR |
| Identifier |
HDL
http://hdl.handle.net/2115/54753
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| Relation |
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isIdenticalTo
DOI
https://doi.org/10.4236/abc.2012.23030
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| Journal |
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Advances in Biological Chemistry
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Volume Number2
Issue Number3
Page Start243
Page End247
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| File |
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| Oaidate |
2023-07-26 |
