Lactobacillus helveticus SBT2171 Inhibits Lymphocyte Proliferation by Regulation of the JNK Signaling Pathway

Hosoya Tomohiro; Sakai Fumihiko; Yamashita Maya; Shiozaki Takuya; Endo Tsutomu; Ukibe Ken; Uenishi Hiroshi; Kadooka Yukio; Moriya Tomohiro; Nakagawa Hisako; Nakayama Yosuke; Miyazaki Tadaaki

Title	en Lactobacillus helveticus SBT2171 Inhibits Lymphocyte Proliferation by Regulation of the JNK Signaling Pathway
Creator	en Hosoya, Tomohiro en Sakai, Fumihiko en Yamashita, Maya en Shiozaki, Takuya en Endo, Tsutomu en Ukibe, Ken en Uenishi, Hiroshi en Kadooka, Yukio en Moriya, Tomohiro en Nakagawa, Hisako en Nakayama, Yosuke en Miyazaki, Tadaaki NRID 1000060272431
Accessrights	open access
Rights	http://creativecommons.org/licenses/by/4.0/deed.ja en Creative Commons Attribution 4.0 International
Subject	NDC 490
Description	Abstract en Lactobacillus helveticus SBT2171 (LH2171) is a lactic acid bacterium with high protease activity and used in starter cultures in the manufacture of cheese. We recently reported that consumption of cheese manufactured using LH2171 alleviated symptoms of dextran sodium sulfate (DSS)-induced colitis in mice. In this study, we have examined whether LH2171 itself exerts an inhibitory effect on the excessive proliferation of lymphocytes. We found that LH2171 inhibited the proliferation of LPS-stimulated mouse T and B cells, and the human lymphoma cell lines, Jurkat and BJAB. Cell cycle analysis showed an accumulation of LH2171-treated BJAB cells in the G2/M phase. Further, phosphorylation of c-Jun N-terminal kinase (JNK) and c-Jun was reduced by LH2171 in BJAB cells. Subsequently, expression of cell division cycle 2 (CDC2), regulated by the JNK signaling pathway and essential for G2/M phase progression, was inhibited by LH2171. It was also demonstrated that intraperitoneal administration of LH2171 strongly alleviated symptoms of collagen-induced arthritis (CIA) in mice. These findings suggest that LH2171 inhibits the proliferation of lymphocytes through a suppression of the JNK signaling pathway and exerts an immunosuppressive effect in vivo.
Publisher	en The Public Library of Science
Date	Issued2014-09-30
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/57673
Relation	isIdenticalTo DOI https://doi.org/10.1371/journal.pone.0108360
Journal	PISSN 1932-6203 en PLOS one Volume Number9 Issue Number9 Page Starte108360
File	fulltext fetchObject.pdf 1012.26 KB (application/pdf) Issued2014-09-30
Oaidate	2023-07-26