Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation

Goto Keiko; Kimura Taichi; Kitamura Nobuto; Semba Shingo; Ohmiya Yoshihiro; Aburatani Sachiyo; Matsukura Satoko; Tsuda Masumi; Kurokawa Takayuki; Gong Jian Ping; Tanaka Shinya; Yasuda Kazunori

Title	en Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation
Creator	en Goto, Keiko en Kimura, Taichi NRID 1000090435959 en Kitamura, Nobuto NRID 1000080447044 en Semba, Shingo NRID 1000040466496 en Ohmiya, Yoshihiro NRID 1000020223951 en Aburatani, Sachiyo NRID 1000010361627 en Matsukura, Satoko en Tsuda, Masumi NRID 1000030431307 en Kurokawa, Takayuki NRID 1000040451439 en Gong, Jian Ping NRID 1000020250417 en Tanaka, Shinya NRID 1000070261287 en Yasuda, Kazunori NRID 1000020166507
Accessrights	open access
Rights	en This is the peer reviewed version of the following article: Goto K, Kimura T, Kitamura N, Semba S, Ohmiya Y, Aburatani S, Matsukura S, Tsuda M, Kurokawa T, Gong JP, Tanaka S, Yasuda K. 2016. Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation. J Biomed Mater Res Part A 2016:104A:734–746., which has been published in final form at http://dx.doi.org/10.1002/jbm.a.35615. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Subject	Other en PAMPS hydrogel Other en chondrogenic differentiation Other en signaling pathway Other en bone morphogenetic protein Other en transforming growth factor Other en BMP Other en TGF NDC 490
Description	Abstract en The purposes of this study were to identify signaling pathways that were specifically activated in ATDC5 cells cultured on poly (2-acrylamido-2-methylpropanesulfonic acid) (PAMPS) gel in insulin-free maintenance medium and to evaluate the significance of the determined signaling pathways in the chondrogenic differentiation induced by this gel. In this study, ATDC5 cells cultured on PAMPS gel using the maintenance medium without insulin (PAMPS Culture) were compared with cells cultured on polystyrene using the differentiation medium containing insulin (PS-I Culture). The microarray analysis, Western blot analysis, and real-time PCR analysis demonstrated that the TGF-β/BMP signaling pathway was significantly enhanced at Days 1, 2, and 3 in the PAMPS Culture when compared with the PS-I Culture. Inhibition of the BMP type-I receptor reduced the phosphorylation level of Smad1/5 and expression of type-2 collagen and aggrecan mRNA in the cells accompanied by a reduction in cell aggregation at Day 13 in the PAMPS Culture. The inhibition of the TGF-β/BMP signaling pathway significantly inhibited the chondrogenic differentiation induced by the PAMPS gel. The present study demonstrated that synthetic PAMPS gel activates the TGF-β/BMP/Smad signaling pathway in the ATDC5 cells in the absence of insulin, and that this activation plays a significant role in the chondrogenic differentiation induced by PAMPS gel.
Publisher	en Wiley-Blackwell
Date	Issued2016-03
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/64638
Relation	isVersionOf DOI https://doi.org/10.1002/jbm.a.35615 PMID 26566602
Journal	PISSN 1549-3296 en Journal of biomedical materials research part A Volume Number104 Issue Number3 Page Start734 Page End746
File	fulltext JBiomedMaterResA104_734.pdf 1.11 MB (application/pdf) Issued2016-03
Oaidate	2023-07-26