Involvement of the nuclear progestin receptor in LH-induced expression of membrane type 2-matrix metalloproteinase required for follicle rupture during ovulation in the medaka, Oryzias latipes

Ogiwara Katsueki; Takahashi Takayuki

Title	en Involvement of the nuclear progestin receptor in LH-induced expression of membrane type 2-matrix metalloproteinase required for follicle rupture during ovulation in the medaka, Oryzias latipes
Creator	en Ogiwara, Katsueki NRID 1000000422006 en Takahashi, Takayuki NRID 1000080197152
Accessrights	open access
Rights	en © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ en Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Subject	Other en Medaka Other en Ovulation Other en LH Other en Mmp15 Other en Nuclear progestin receptor NDC 460
Description	Abstract en Hormonal regulation of the expression of Mmp15, a proteolytic enzyme indispensable for ovulation in the teleost medaka, was investigated. In an in vitro culture system using preovulatory follicles, Mmp15 expression and ovulation were induced in the presence of recombinant luteinizing hormone (rLh). Both rLh-induced Mmp15 expression and ovulation were 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one-dependent, suggesting the involvement of a nuclear progestin receptor (Pgr). In vitro follicle ovulation and Mmp15 expression were reduced by treatment with the Pgr antagonist RU-486. Like Pgr, the transcription factor CCAAT/enhancer-binding protein beta (Cebpb) was induced by rLh. ChIP analyses indicated that Pgr and Cebpb bound to the mmpl5 promoter region. These results indicate that the rLh-induced expression of Mmp15 is mediated by Pgr and Cebpb. A differential timing of expression of Pgr and Cebpb in the preovulatory follicles appears to explain the considerably long time-lag from the pgr gene activation to mmpl5 gene expression. (C) 2017 Elsevier B.V. All rights reserved.
Publisher	en Elsevier
Date	Issued2017-07-15
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/68833
Relation	isVersionOf DOI https://doi.org/10.1016/j.mce.2017.04.016 PMID 28416325
Journal	PISSN 0303-7207 en Molecular and cellular endocrinology Volume Number450 Page Start54 Page End63
File	fulltext Ogiwara et al., 2017 MCE.pdf 5.85 MB (application/pdf) Issued2017-07-15
Oaidate	2023-07-26