| Title |
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en
Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi
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| Creator |
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| Accessrights |
metadata only access |
| Rights |
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| Subject |
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Other
en
Food science
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Other
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Functional foods
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Other
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Protein glycation
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Other
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Immune response
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Other
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Glycation
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Other
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Larix kaempferi
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Other
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Taxifolin
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Other
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Flavonoids
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Other
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Dihydroquercetin
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NDC
498
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| Description |
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Abstract
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The larches, the Larix genus of plants are known as a natural source of taxifolin (dihydroquercetin), and extracts of its taxifolin rich xylem are used in dietary supplements to maintain health. In the present study, to assess biological activities of a methanol extract of the Japanese larch, Larix kaempferi (LK-ME), the effects of LK-ME on cell viability, inflammatory cytokine expression, and glycation were investigated. The effects of taxifolin which is known to be a main compound of LK-ME, and its related flavonoids, quercetin and luteolin were also examined. The results show that taxifolin exhibits lower growth inhibition activity and lesser induction activity of inflammatory cytokines in a human monocyte derived cell line, THP-1 cells, while in vitro anti-glycation activities of taxifolin were inhibiting at comparable levels to those of quercetin and luteolin. The growth inhibition and the cytokine induction activities, and the anti-glycation effects of LK-ME are assumed to have properties similar to taxifolin. The results of high performance liquid chromatography (HPLC) analysis indicated that taxifolin was detected as the main peak of LK-ME at the absorbance of 280 nm, and the concentration of taxifolin was measured as 3.12 mg/ml. The actual concentration of taxifolin in LK-ME is lower than the concentration estimated from the IC50 values calculated by the results of glycation assays, suggesting that other compounds contained in LK-ME are involved in the anti-glycation activity.
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| Publisher |
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Elsevier
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| Date |
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| Language |
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| Resource Type |
journal article |
| Version Type |
NA |
| Identifier |
HDL
http://hdl.handle.net/2115/75276
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| Relation |
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isIdenticalTo
DOI
https://doi.org/10.1016/j.heliyon.2019.e02047
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| Journal |
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Heliyon
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Volume Number5
Issue Number7
Page Starte02047
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| Oaidate |
2023-07-26 |
