Immunolocalization of podoplanin/E11/gp38, CD44, and endomucin in the odontoblastic cell layer of murine tooth germs

Khadiza Naznin; Hasegawa Tomoka; Nagai Tomoya; Yamamoto Tomomaya; Miyamoto-Takasaki Yukina; Hongo Hiromi; Abe Miki; Haraguchi Mai; Yamamoto Tsuneyuki; Yi Min; Qiu Zixuan; Sasaki Muneteru; Kuroshima Shinichiro; Ohshima Hayato; Luiz de Freitas Paulo Henrique; Li Minqi; Yawaka Yasutaka; Amizuka Norio

Title	en Immunolocalization of podoplanin/E11/gp38, CD44, and endomucin in the odontoblastic cell layer of murine tooth germs
Creator	en Khadiza, Naznin en Hasegawa, Tomoka NRID 1000050739349 en Nagai, Tomoya en Yamamoto, Tomomaya en Miyamoto-Takasaki, Yukina en Hongo, Hiromi NRID 1000000778970 en Abe, Miki en Haraguchi, Mai en Yamamoto, Tsuneyuki NRID 1000080200822 en Yi, Min NRID 1000040292007 en Qiu, Zixuan en Sasaki, Muneteru en Kuroshima, Shinichiro en Ohshima, Hayato en Luiz de Freitas, Paulo Henrique en Li, Minqi en Yawaka, Yasutaka NRID 1000060230603 en Amizuka, Norio NRID 1000030242431
Accessrights	metadata only access
Subject	NDC 497
Description	Abstract en In this study, we attempted to localize the immunoreactivities of podoplanin/E11/gp38 and CD44, a counterpart possessing a high affinity to podoplanin/E11/gp38, as well as endomucin-immunoreactive blood vessels in the regions of odontoblast layers and the underlying sub-odontoblastic layers in murine tooth germs. Endomucin-reactive small blood vessels were scattered throughout the dental papillae of the tooth germs at postnatal day 1 but came to be localized close to the odontoblast/sub-odontoblastic layers until day 3. After postnatal day 5, small blood vessels were seen in odontoblast cell layers, while blood vessels with relatively larger diameters were seen forming in sub-odontoblastic layers. Immunoreactivities of podoplanin/E11/gp38 and CD44 were not detectable in the cells of dental papillae facing the inner enamel epithelium at postnatal day 1. However, at around postnatal days 3-5, podoplanin/E11/gp38 was localized in the odontoblast layer but not in the sub-odontoblastic layer, whereas CD44 was observed in the sub-odontoblastic layer but not in the odontoblast layer. The exclusive immunolocalization of podoplanin/E11/gp38 and CD44 in the odontoblast layers and sub-odontoblastic layers was seen after postnatal day 3 of the tooth germs, when the mesenchymal cells of dental papillae have already differentiated into mature odontoblasts at the cusp tip. Taken together, it seems likely that endomucin-reactive small blood vessels extended to the podoplanin/E11/gp38-positive odontoblast layers, whereas endomucin-reactive large blood vessels were already present in CD44-immmunopositive sub-odontoblastic layer, indicating the cellular regulation on the vascularization of endomucin-reactive endothelial cells during odontogenesis of the tooth germs.
Publisher	en Biomedical Research Press
Date	Issued2019-08-01
Language	eng
Resource Type	journal article
Version Type	NA
Identifier	HDL http://hdl.handle.net/2115/75445
Relation	isIdenticalTo DOI https://doi.org/10.2220/biomedres.40.133
Journal	PISSN 0388-6107 en Biomedical Research (Tokyo) Volume Number40 Issue Number4 Page Start133 Page End143
Oaidate	2023-07-26