Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses

Sakurai Akira; Takayama Katsuyoshi; Nomura Namiko; Kajiwara Naoki; Okamatsu Masatoshi; Yamamoto Naoki; Tamura Tsuruki; Yamada Jitsuho; Hashimoto Masako; Sakoda Yoshihiro; Suda Yoshihiko; Kobayashi Yukuharu; Kida Hiroshi; Shibasaki Futoshi

Title	en Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses
Creator	en Sakurai, Akira en Takayama, Katsuyoshi en Nomura, Namiko NRID 1000050599694 en Kajiwara, Naoki NRID 1000070453917 en Okamatsu, Masatoshi NRID 1000000507163 en Yamamoto, Naoki en Tamura, Tsuruki en Yamada, Jitsuho en Hashimoto, Masako en Sakoda, Yoshihiro NRID 1000040333637 en Suda, Yoshihiko en Kobayashi, Yukuharu en Kida, Hiroshi NRID 1000010109506 en Shibasaki, Futoshi NRID 1000090300954
Accessrights	metadata only access
Rights	https://creativecommons.org/licenses/by/4.0/ en Creative Commons Attribution 4.0 International
Subject	NDC 493
Description	Abstract en Lateral flow tests also known as Immunochromatography (IC) is an antigen-detection method conducted on a nitrocellulose membrane that can be completed in less than 20 min. IC has been used as an important rapid test for clinical diagnosis and surveillance of influenza viruses, but the IC sensitivity is relatively low (approximately 60%) and the limit of detection (LOD) is as low as 10³ pfu per reaction. Recently, we reported an improved IC assay using antibodies conjugated with fluorescent beads (fluorescent immunochromatography; FLIC) for subtyping H5 influenza viruses (FLIC-H5). Although the FLIC strip must be scanned using a fluorescent reader, the sensitivity (LOD) is significantly improved over that of conventional IC methods. In addition, the antibodies which are specific against the subtypes of influenza viruses cannot be available for the detection of other subtypes when the major antigenicity will be changed. In this study, we established the use of FLIC to type seasonal influenza A and B viruses (FLIC-AB). This method has improved sensitivity to 100-fold higher than that of conventional IC methods when we used several strains of influenza viruses. In addition, FLIC-AB demonstrated the ability to detect influenza type A and influenza type B viruses from clinical samples with high sensitivity and specificity (Type A: sensitivity 98.7% (74/75), specificity 100% (54/54), Type B: sensitivity 100% (90/90), specificity 98.2% (54/55) in nasal swab samples) in comparison to the results of qRT-PCR. And furthermore, FLIC-AB performs better in the detection of early stage infection (under 13h) than other conventional IC methods. Our results provide new strategies to prevent the early-stage transmission of influenza viruses in humans during both seasonal outbreaks and pandemics.
Publisher	en Public Library of Science
Date	Issued2015-02-04
Language	eng
Resource Type	journal article
Version Type	NA
Identifier	HDL http://hdl.handle.net/2115/75587
Relation	isIdenticalTo DOI https://doi.org/10.1371/journal.pone.0116715 PMID 25650570
Journal	EISSN 1932-6203 en PLoS One Volume Number10 Issue Number2 Page Starte0116715
Oaidate	2023-07-26