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Title	en Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan
Creator	en Abe, Yuri en Tamura, Tomokazu en Torii, Shiho en Wakamori, Shiho en Nagai, Makoto en Mitsuhashi, Kazuya en Mine, Junki en Fujimoto, Yuri en Nagashima, Naofumi en Yoshino, Fumi en Sugita, Yukihiko en Nomura, Takushi en Okamatsu, Masatoshi NRID 1000000507163 en Kida, Hiroshi NRID 1000010109506 en Sakoda, Yoshihiro NRID 1000040333637
Accessrights	metadata only access
Rights	https://creativecommons.org/licenses/by-nc-nd/4.0/ en Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Subject	Other en antigenicity Other en bovine viral diarrhea virus Other en E2 Other en genetic diversity NDC 649
Description	Abstract en In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs) isolated from 2000 to 2006 in Japan and reported that subgenotype 1b viruses were predominant. In the present study, 766 BVDVs isolated from 2006 to 2014 in Hokkaido, Japan, were genetically analyzed to understand recent epidemics. Phylogenetic analysis based on nucleotide sequences of the 5′-untranslated region of viral genome revealed that 766 isolates were classified as genotype 1 (BVDV-1; 544 isolates) and genotype 2 (BVDV-2; 222). BVDV-1 isolates were further divided into BVDV-1a (93), 1b (371) and 1c (80) subgenotypes, and all BVDV-2 isolates were grouped into BVDV-2a subgenotype (222). Further comparative analysis was performed with BVDV-1a, 1b and 2a viruses isolated from 2001 to 2014. Phylogenetic analysis based on nucleotide sequences of the viral glycoprotein E2 gene, a major target of neutralizing antibodies, revealed that BVDV-1a, 1b and 2a isolates were further classified into several clusters. Cross-neutralization tests showed that BVDV-1b isolates were antigenically different from BVDV-1a isolates, and almost BVDV-1a, 1b and 2a isolates were antigenically similar among each subgenotype and each E2 cluster. Taken together, BVDV-1b viruses are still predominant, and BVDV-2a viruses have increased recently in Hokkaido, Japan. Field isolates of BVDV-1a, 1b and 2a show genetic diversity on the E2 gene with antigenic conservation among each subgenotype during the last 14 years.
Publisher	en The Japanese Society of Veterinary Science ja 日本獣医学会
Date	Issued2016-01
Language	eng
Resource Type	journal article
Version Type	NA
Identifier	HDL http://hdl.handle.net/2115/76355
Relation	isIdenticalTo DOI https://doi.org/10.1292/jvms.15-0186 PMID 26400674
Journal	PISSN 0916-7250 EISSN 1347-7439 NCID AA10796138 en Journal of Veterinary Medical Science Volume Number78 Issue Number1 Page Start61 Page End70
Oaidate	2023-07-26