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Title
  • en Characterization and expression profiles of small heat shock proteins in the marine red alga Pyropia yezoensis
Creator
Accessrights open access
Rights
  • en This is a post-peer-review, pre-copyedit version of an article published in Cell Stress and Chaperones. The final authenticated version is available online at: http://dx.doi.org/10.1007/s12192-018-00959-9
Subject
  • Other en Pyropia yezoensis
  • Other en Small heat shock proteins
  • Other en Red algae
  • Other en Abiotic stress
  • Other en Plant growth hormone
  • NDC 474
Description
  • Abstract en Small heat shock proteins (sHSPs) are found in all three domains of life (Bacteria, Archaea, and Eukarya) and play a critical role in protecting organisms from a range of environmental stresses. However, little is known about their physiological functions in red algae. Therefore, we characterized the sHSPs (PysHSPs) in the red macroalga Pyropia yezoensis, which inhabits the upper intertidal zone where it experiences fluctuating stressful environmental conditions on a daily and seasonal basis, and examined their expression profiles at different developmental stages and under varying environmental conditions. We identified five PysHSPs (PysHSP18.8, 19.1, 19.2, 19.5, and 25.8). Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that expression of the genes PysHSP18.8, PysHSP19.5, and PysHSP25.8 was repressed at all the developmental stages under normal conditions, whereas PysHSP19.1 and PysHSP19.2 were overexpressed in mature gametophytes and sporophytes. Exposure of the gametophytes to high temperature, oxidative stress, or copper significantly increased the mRNA transcript levels of all the five genes, while exogenous application of the ethylene precursor 1-aminocylopropane-1-carboxylic acid (ACC) significantly increased the expression levels of PysHSP19.2, PysHSP19.5, and PysHSP25.8. These findings will help to further our understanding of the role of PysHSP genes and provide clues about how Pyropia species can adapt to the stressful conditions encountered in the upper intertidal zone during their life cycle.
Publisher en Springer
Date
    Issued2019-01
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/76496
Relation
  • isVersionOf DOI https://doi.org/10.1007/s12192-018-00959-9
Journal
    • PISSN 1355-8145
    • EISSN 1466-1268
      • en Cell Stress and Chaperones
      • Volume Number24 Issue Number1 Page Start223 Page End233
File
Oaidate 2023-07-26