Efficient isolation of Swine influenza viruses by age-targeted specimen collection

Ozawa Makoto; Matsuu Aya; Yonezawa Kouki; Igarashi Manabu; Okuya Kosuke; Kawabata Toshiko; Ito Kimihito; Kohara Kyoko; Taneno Akira; Deguchi Eisaburo

Title	en Efficient isolation of Swine influenza viruses by age-targeted specimen collection
Creator	en Ozawa, Makoto NRID 1000050568722 en Matsuu, Aya NRID 1000040348595 en Yonezawa, Kouki NRID 1000000374744 en Igarashi, Manabu NRID 1000010374240 en Okuya, Kosuke en Kawabata, Toshiko en Ito, Kimihito NRID 1000060396314 en Kohara, Kyoko NRID 1000020225478 en Taneno, Akira en Deguchi, Eisaburo
Accessrights	metadata only access
Subject	NDC 649
Description	Abstract en The control of swine influenza virus (SIV) infection is paramount for increasing the productivity of pig farming and minimizing the threat of pandemic outbreaks. Thus, SIV surveillance should be conducted by region and on a regular basis. Here, we established a microneutralization assay specific for SIV seroprevalence surveillance by using reporter gene-expressing recombinant influenza viruses. Growth-based SIV seroprevalence revealed that most sows and piglets were positive for neutralizing antibodies against influenza viruses. In contrast, the 90-day-old growing pigs exhibited limited neutralizing activity in their sera, suggesting that this particular age of population is most susceptible to SIV infection and thus is an ideal age group for SIV isolation. From nasal swab specimens of healthy pigs in this age population, we were able to isolate SIVs at a higher incidence (5.3%) than those of previous reports. Nucleotide sequencing and phylogenetic analysis of the hemagglutinin (HA) genes revealed that the isolated SIVs have circulated and evolved in pigs but not have been recently introduced from humans, implying that a large number of SIV lineages may remain “undiscovered” in the global porcine populations. We propose that the 90-day-old growing pig-targeted nasal swab collection presented in this study facilitates global SIV surveillance and contributes to the detection and control of SIV infection.
Publisher	en American Society for Microbiology
Date	Issued2015-04
Language	eng
Resource Type	journal article
Version Type	NA
Identifier	HDL http://hdl.handle.net/2115/76879
Relation	isIdenticalTo DOI https://doi.org/10.1128/JCM.02941-14 PMID 25694523
Journal	PISSN 0095-1137 EISSN 1098-660X NCID AA00695531 en Journal Clinical Microbiology Volume Number53 Issue Number4 Page Start1331 Page End1338
Oaidate	2023-07-26