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Title	en Expression profiling of sexually dimorphic genes in the Japanese quail, Coturnix japonica
Creator	en Okuno, Miki en Miyamoto, Shuntaro en Itoh, Takehiko en Seki, Masahide en Suzuki, Yutaka en Mizushima, Shusei en Kuroiwa, Asato NRID 1000020372261
Accessrights	metadata only access
Subject	NDC 467
Description	Abstract en Research on avian sex determination has focused on the chicken. In this study, we established the utility of another widely used animal model, the Japanese quail (Coturnix japonica), for clarifying the molecular mechanisms underlying gonadal sex differentiation. In particular, we performed comprehensive gene expression profiling of embryonic gonads at three stages (HH27, HH31 and HH38) by mRNA-seq. We classified the expression patterns of 4,815 genes into nine clusters according to the extent of change between stages. Cluster 2 (characterized by an initial increase and steady levels thereafter), including 495 and 310 genes expressed in males and females, respectively, contained five key genes involved in gonadal sex differentiation. A GO analysis showed that genes in this cluster are related to developmental processes including reproductive structure development and developmental processes involved in reproduction were significant, suggesting that expression profiling is an effective approach to identify novel candidate genes. Based on RNA-seq data and in situ hybridization, the expression patterns and localization of most key genes for gonadal sex differentiation corresponded well to those of the chicken. Our results support the effectiveness of the Japanese quail as a model for studies gonadal sex differentiation in birds.
Publisher	en Nature Research
Date	Issued2020-11-30
Language	eng
Resource Type	journal article
Version Type	NA
Identifier	HDL http://hdl.handle.net/2115/80565
Relation	isIdenticalTo DOI https://doi.org/10.1038/s41598-020-77094-y
Journal	PISSN 2045-2322 en Scientific reports Volume Number10 Issue Number1 Page Start20073
Oaidate	2023-07-26