The Ameliorative Effect of Dexamethasone on the Development of Autoimmune Lung Injury and Mediastinal Fat-Associated Lymphoid Clusters in an Autoimmune Disease Mouse Model

Elewa Yaser Hosny Ali; Masum Md Abdul; Mohamed Sherif Kh A.; Islam Md Rashedul; Nakamura Teppei; Ichii Osamu; Kon Yasuhiro

Title	en The Ameliorative Effect of Dexamethasone on the Development of Autoimmune Lung Injury and Mediastinal Fat-Associated Lymphoid Clusters in an Autoimmune Disease Mouse Model
Creator	en Elewa, Yaser Hosny Ali ORCID 0000-0002-7347-4587 en Masum, Md Abdul en Mohamed, Sherif Kh A. en Islam, Md Rashedul en Nakamura, Teppei NRID 1000080786773 en Ichii, Osamu NRID 1000060547769 en Kon, Yasuhiro NRID 1000010178402
Accessrights	metadata only access
Rights	https://creativecommons.org/licenses/by/4.0/ en Creative Commons Attribution 4.0 International
Subject	Other en autoimmune disease mouse model Other en dexamethasone Other en high endothelial venules Other en lung injury Other en lymphatic vessels Other en mediastinal fat-associated lymphoid cluster Other en proliferating cells NDC 649
Description	Abstract en In our previous study, we revealed the ameliorative therapeutic effect of dexamethasone (Dex) for Lupus nephritis lesions in the MRL/MpJ-Fas (lpr/lpr) (Lpr) mouse model. The female Lpr mice developed a greater number of mediastinal fat-associated lymphoid clusters (MFALCs) and inflammatory lung lesions compared to the male mice. However, the effect of Dex, an immunosuppressive drug, on both lung lesions and the development of MFALCs in Lpr mice has not been identified yet. Therefore, in this study, we compared the development of lung lesions and MFALCs in female Lpr mice that received either saline (saline group "SG") or dexamethasone (dexamethasone group "DG") in drinking water as a daily dose along with weekly intraperitoneal injections for 10 weeks. Compared to the SG group, the DG group showed a significant reduction in the levels of serum anti-dsDNA antibodies, the size of MFALCs, the degree of lung injury, the area of high endothelial venules (HEVs), and the number of proliferating and immune cells in both MFALCs and the lungs. A significant positive correlation was observed between the size of MFALCs and the cellular aggregation in the lungs of Lpr mice. Therefore, this study confirmed the ameliorative effect of Dex on the development of lung injury and MFALCs via their regressive effect on both immune cells' proliferative activity and the development of HEVs. Furthermore, the reprogramming of MFALCs by targeting immune cells and HEVs may provide a therapeutic strategy for autoimmune-disease-associated lung injury.
Publisher	en MDPI
Date	Issued2022-04-18
Language	eng
Resource Type	journal article
Version Type	NA
Identifier	HDL http://hdl.handle.net/2115/85601
Relation	isIdenticalTo DOI https://doi.org/10.3390/ijms23084449
Journal	PISSN 1422-0067 en International Journal of Molecular Sciences Volume Number23 Issue Number8 Page Start4449
Oaidate	2023-07-26