In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens

Fujisawa Sotaro; Murata Shiro; Takehara Masaki; Aoyama Julia; Morita Ayu; Isezaki Masayoshi; Win Shwe Yee; Ariizumi Takuma; Sato Takumi; Oishi Eiji; Taneno Akira; Maekawa Naoya; Okagawa Tomohiro; Ichii Osamu; Konnai Satoru; Ohashi Kazuhiko

Title	en In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens
Creator	en Fujisawa, Sotaro en Murata, Shiro NRID 1000010579163 en Takehara, Masaki en Aoyama, Julia en Morita, Ayu en Isezaki, Masayoshi en Win, Shwe Yee en Ariizumi, Takuma en Sato, Takumi en Oishi, Eiji en Taneno, Akira en Maekawa, Naoya NRID 1000070829035 en Okagawa, Tomohiro NRID 1000080829036 en Ichii, Osamu NRID 1000060547769 en Konnai, Satoru NRID 1000040396304 en Ohashi, Kazuhiko NRID 1000090250498
Accessrights	open access
Rights	en © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ en Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Subject	Other en Poultry red mite Other en Adipocyte plasma membrane-associated protein Other en Vaccine Other en Dg-APMAP Other en Dermanyssus gallinae NDC 649
Description	Abstract en The poultry red mite (Dermanyssus gallinae; PRM) is a blood-sucking ectoparasite of chickens that is a threat to poultry farming worldwide and significantly reduces productivity in the egg-laying industry. Chemical acaricides that are widely used in poultry farms for the prevention of PRMs are frequently ineffective due to the emergence of acaricide-resistant PRMs. Therefore, alternative control methods are needed, and vaccination is a promising strategy for controlling PRMs. A novel adipocyte-plasma membrane-associated protein-like molecule (Dg-APMAP) is highly expressed in blood-fed PRMs according to a previous RNA sequencing analysis. Here, we attempted to identify the full sequence of DgAPMAP, study its expression in different life stages of PRMs, and evaluate its potential as a vaccine antigen. Dg-APMAP mRNA was expressed in the midgut and ovaries, and in all life stages regardless of feeding states. Importantly, in vitro feeding of PRMs with plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-APMAP significantly reduced their survival rate in nymphs and adults, which require blood meals. Our data suggest that the host immune responses induced by vaccination with Dg-APMAP could be an effective strategy to reduce the suffering caused by PRMs in the poultry industry. (c) 2021 Elsevier Ltd. All rights reserved.
Publisher	en Elsevier
Date	Issued2021-10-01
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/86867
Relation	isVersionOf DOI https://doi.org/10.1016/j.vaccine.2021.08.104
Journal	PISSN 0264-410X en Vaccine Volume Number39 Issue Number41 Page Start6057 Page End6066
File	fulltext Manuscript.pdf 899.84 KB (application/pdf) Issued2021-10-01 fulltext Supplementary materials.pptx 166.53 KB (application/vnd.openxmlformats-officedocument.presentationml.presentation) Issued2021-10-01
Oaidate	2023-07-26