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Title
  • Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: Simultaneous measurements of phototactic and photophobic responses
  • en Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: Simultaneous measurements of phototactic and photophobic responses
Creator
    • 渡辺, 正勝
    • e-Rad 40124226
    • TAKAHASHI, Tetsuo
    • YOSHIHARA, Kazuo
    • WATANABE, Masakatsu
    • KUBOTA, Mamoru
    • JOHNSON, Randy
    • DERGUINI, Fadila
    • NAKANISHI, Koji
Accessrights metadata only access
Rights
  • © 1991 Published by Elsevier Inc.
Description
  • Abstract A real-time automated method was developed for simultaneous measurements of phototactic orientation (phototaxis) and step-up photophobic response of flagellated microorganisms. Addition of all-trans retinal restored both photoresponses in a carotenoid-deficient mutant strain of Chlamydomonas reinhardtii in a dose-dependent manner. The phototactic orientation was biphasic with respect to both the light intensity and the concentration of retinal. All-trans retinal was more effective than 11-cis retinal to regenerate both photobehavioral responses. Analogs having locked 11-cis configurations and a phenyl ring in the side chain also induced photoresponses, although at concentrations more than two orders of magnitude higher than all-trans retinal. According to the present assay method, the responses were hardly detectable in cells incubated with retinal analogs in which the 13-ene was locked in either its trans or cis configuration. The results strongly suggest that the isomerization of the 13-14 double bond is important for photobehavioral signal transduction and that a single retinal-dependent photoreceptor controls both phototactic and photophobic responses.
Publisher Elsevier
Date
    Issued1991-08-15
Language
  • eng
Resource Type journal article
Identifier URI https://ir.soken.ac.jp/records/3862
Relation
  • PMID 1872847
  • DOI https://doi.org/10.1016/0006-291X(91)91031-7 10.1016/0006-291X(91)91031-7
Journal
    • ISSN 0006291X
      • Biochemical and Biophysical Research Communications en Biochemical and Biophysical Research Communications
      • Volume Number178 Issue Number3 Page Start1273 Page End1279
Oaidate 2023-08-18