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Title
  • en DJ-1, a Target Protein for an Endocrine Disrupter, Participates in the Fertilization in Mice
Creator
Accessrights open access
Subject
  • Other en DJ-1
  • Other en fertilization
  • Other en endocrine disrupter
  • NDC 499
Description
  • Abstract en DJ-1 was first identified as an activated ras-dependent oncogene product and was later also found to be an infertility-related protein affected by sperm toxicants such as ornidazole (OR) and epichlorohydrin. These findings suggest that DJ-1 has functions in both somatic cells and sperm. In this study, to determine the relationship between DJ-1 and an endocrine disrupter and to determine the functions of DJ-1 in sperm, in vitro fertilization experiments were carried out using eggs and sperm extracted from mice that had or had not been treated with OR. We found that the amount of DJ-1 in sperm and the efficiency of fertilization decreased with the increasing dose of OR to which the mice were exposed. The addition of an anti-mouse DJ-1 serum to sperm solution before the in vitro fertilization reaction with eggs resulted in a decrease in the efficiency of fertilization to about one-third of that when pre-immune serum was added to sperm solution, indicating that DJ-1 participates in the fertilization.
Publisher en The Pharmaceutical Society of Japan
Date
    Issued2002-07
Language
  • eng
Resource Type journal article
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/53744
Relation
  • isIdenticalTo DOI https://doi.org/10.1248/bpb.25.853
  • PMID 12132656
Journal
    • PISSN 0918-6158
    • EISSN 1347-5215
      • en Biological & Pharmaceutical Bulletin
      • Volume Number25 Issue Number7 Page Start853 Page End856
File
Oaidate 2023-07-26