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Title
  • en Polar localization and endocytic degradation of a boron transporter, BOR1, is dependent on specific tyrosine residues
Creator
    • en Yoshinari, Akira
    • en Kasai, Koji
    • en Fujiwara, Toru
    • en Takano, Junpei
Accessrights open access
Subject
  • Other en mineral transporter
  • Other en polar localization
  • Other en tyrosine-based signal
  • Other en ubiquitination
  • Other en membrane trafficking
  • Other en endocytosis
  • NDC 460
Description
  • Abstract en Boron (B) is essential for plants, but is toxic in excess. Plants have to strictly regulate the uptake and translocation of B. In Arabidopsis thaliana root cells, a boric acid channel, NIP5;1, and a boric acid/borate exporter, BOR1, localize to the outer (facing soil) and inner plasma membrane domains, respectively, under B limitation. The opposite polar localizations of the importer and exporter would enable plant roots to transport B efficiently towards the xylem. In addition, accumulation of the B transporters is controlled by B conditions. When plants are shifted from low to high B conditions, NIP5;1 transcript accumulation is down-regulated through mRNA degradation. The BOR1 protein is transported to the trans-Golgi network/early endosome and multivesicular body and finally degraded in the vacuole. We have recently shown that both the polar localization and the endocytic degradation of BOR1 are controlled by at least two tyrosine residues in a large loop located in the cytosol. We also showed that ubiquitination is required for the endocytic degradation of BOR1. Here, we analyzed possible involvement of an additional tyrosine residue (Y414) in the loop region and discuss the pathway of the BOR1 trafficking for polar localization and endocytic degradation of BOR1.
Publisher en Taylor & Francis
Date
    Issued2012-01
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/59503
Relation
  • isVersionOf DOI https://doi.org/10.4161/psb.7.1.18527
Journal
    • EISSN 1559-2324
      • en Plant Signaling & Behavior
      • Volume Number7 Issue Number1 Page Start46 Page End49
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Oaidate 2023-07-26