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Title	en The RNA Sensor RIG-I Dually Functions as an Innate Sensor and Direct Antiviral Factor for Hepatitis B Virus
Alternative	en RIG-I functions as a dual effector against HBV
Creator	en Sato, Seiichi en Li, Kai en Kameyama, Takeshi NRID 1000040569505 en Hayashi, Takaya en Ishida, Yuji en Murakami, Shuko NRID 1000050454848 en Watanabe, Tsunamasa NRID 1000020338528 en Iijima, Sayuki NRID 1000000416273 en Sakurai, Yu en Watashi, Koichi NRID 1000040378948 en Tsutsumi, Susumu en Sato, Yusuke NRID 1000010735624 en Akita, Hidetaka NRID 1000080344472 en Wakita, Takaji NRID 1000040280789 en Rice, CharlesM. en Harashima, Hideyoshi NRID 1000000183567 en Kohara, Michinori NRID 1000010250218 en Tanaka, Yasuhito NRID 1000090336694 en Takaoka, Akinori NRID 1000030323611
Accessrights	open access
Rights	en © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ en Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Subject	NDC 490
Description	Other en Supplemental materials are available on the publisher's website. Abstract en Host innate recognition triggers key immune responses for viral elimination. The sensing mechanism of hepatitis B virus (HBV), a DNA virus, and the subsequent downstream signaling events remain to be fully clarified. Here we found that type III but not type I interferons are predominantly induced in human primary hepatocytes in response to HBV infection, through retinoic acid-inducible gene-I (RIG-I)-mediated sensing of the 5'-ε region of HBV pregenomic RNA. In addition, RIG-I could also counteract the interaction of HBV polymerase (P protein) with the 5'-ε region in an RNA-binding dependent manner, which consistently suppressed viral replication. Liposome-mediated delivery and vector-based expression of this ε region-derived RNA in liver abolished the HBV replication in human hepatocyte-chimeric mice. These findings identify an innate recognition mechanism by which RIG-I dually functions as an HBV sensor activating innate signaling and to counteract viral polymerase in human hepatocytes.
Publisher	en Cell Press
Date	Issued2015-01-20
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/62620
Relation	isVersionOf DOI https://doi.org/10.1016/j.immuni.2014.12.016 PMID 25557055
Journal	PISSN 1074-7613 en Immunity Volume Number42 Issue Number1 Page Start123 Page End132
File	fulltext Immunity42_123.pdf 3.78 MB (application/pdf) Issued2015-01-20
Oaidate	2023-07-26