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Title
  • The RNA Sensor RIG-I Dually Functions as an Innate Sensor and Direct Antiviral Factor for Hepatitis B Virus
Alternative
  • RIG-I functions as a dual effector against HBV
Creator

Sato, Seiichi

Li, Kai

Kameyama, Takeshi

Hayashi, Takaya

Ishida, Yuji

Murakami, Shuko

Watanabe, Tsunamasa

Iijima, Sayuki

Sakurai, Yu

Watashi, Koichi

Tsutsumi, Susumu

Sato, Yusuke

Akita, Hidetaka

Wakita, Takaji

Rice, CharlesM.

Harashima, Hideyoshi

Kohara, Michinori

Tanaka, Yasuhito

Takaoka, Akinori

Rights
    • © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
    • http://creativecommons.org/licenses/by-nc-nd/4.0/
Subject
  • NDC 490
Description
Other
  • Host innate recognition triggers key immune responses for viral elimination. The sensing mechanism of hepatitis B virus (HBV), a DNA virus, and the subsequent downstream signaling events remain to be fully clarified. Here we found that type III but not type I interferons are predominantly induced in human primary hepatocytes in response to HBV infection, through retinoic acid-inducible gene-I (RIG-I)-mediated sensing of the 5'-ε region of HBV pregenomic RNA. In addition, RIG-I could also counteract the interaction of HBV polymerase (P protein) with the 5'-ε region in an RNA-binding dependent manner, which consistently suppressed viral replication. Liposome-mediated delivery and vector-based expression of this ε region-derived RNA in liver abolished the HBV replication in human hepatocyte-chimeric mice. These findings identify an innate recognition mechanism by which RIG-I dually functions as an HBV sensor activating innate signaling and to counteract viral polymerase in human hepatocytes.
PublisherCell Press
Date Issued 2015-01-20
Languageeng
NIItypejournal article
VersiontypeAM
Identifier URI http://hdl.handle.net/2115/62620
Relation
  • isIdenticalTo PMID 25557055
  • isIdenticalTo DOI https://doi.org/10.1016/j.immuni.2014.12.016
Journal
    • ISSN 1074-7613
    • Immunity
    42(1), 123-132
File
Oaidate2019-03-15T05:20:29Z